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COL1A1 Antibody / Collagen I

Collagen type I alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

P02452

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

An amino acid sequence from the C-terminus of human Collagen I (QPPQEKAHDGGRYYRA) was used as the immunogen for this COL1A1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This COL1A1 antibody is available for research use only.

Storage Conditions

After reconstitution, the COL1A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the COL1A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human spleen tissue with COL1A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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