Heme Oxygenase 1 Antibody / HO-1 / HMOX1

Heme oxygenase 1, also called HMOX1 and HO-1, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. HO-1 activity is induced by its substrate heme and by various nonheme substances.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Mouse partial recombinant protein (AA 2-261) was used as the immunogen for this Heme Oxygenase 1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Heme Oxygenase 1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Heme Oxygenase 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Heme Oxygenase 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC staining of FFPE mouse liver tissue with Heme Oxygenase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.