Glutathione Reductase Antibody / GSR

Glutathione reductase (GR), also known as glutathione-disulfide reductase (GSR), is an enzyme that in humans is encoded by the GSR gene. This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P00390

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids K256-R522 was used as the immunogen for the Glutathione Reductase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P, IF, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Glutathione Reductase antibody is available for research use only.

Storage Conditions

After reconstitution, the Glutathione Reductase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Glutathione Reductase antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Western blot testing of human 1) HeLa, 2) HepG2, 3) A549 and 4) 22RV1 cell lysate with Glutathione Reductase antibody at 0.5ug/ml. Predicted molecular weight ~55 kDa.