FHIT Antibody

Bis (5'-adenosyl) -triphosphatase, also known as fragile histidine triad protein (FHIT) is an enzyme that in humans is encoded by the FHIT gene. This gene, a member of the histidine triad gene family, encodes a diadenosine P1, P3-bis (5'-adenosyl) -triphosphate adenylohydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3p14.2, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Furthermore, FHIT has been shown to synergize with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. It also acts as a tumor suppressor of HER2/neu driven breast cancer.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P49789

Host

Mouse

Reactivity

Human

Immunogen

Human FHIT recombinant protein (amino acids M1-Q147) was used as the immunogen for the FHIT antibody antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

26H7

Applications

WB, IF, FACS

Purity

Protein G affinity

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Limitations

This FHIT antibody is available for research use only.

Storage Conditions

After reconstitution, the FHIT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the FHIT antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

Immunofluorescent staining of FFPE human MCF7 cells with FHIT antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.