ARAF Antibody / A-Raf

Serine/threonine-protein kinase A-Raf or simply A-Raf is an enzyme that in humans is encoded by the ARAF gene. It is mapped to Xp11.3. This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P10398

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids E309-T413 from the human protein were used as the immunogen for the ARAF antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Limitations

This ARAF antibody is available for research use only.

Storage Conditions

Store the ARAF antibody at -20°C.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ARAF antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human MCF7 cells with ARAF antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.