MTOR Antibody

The mammalian target of rapamycin (mTOR), also known as the mechanistic target of rapamycin and FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase that in humans is encoded by the MTOR gene. The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P42345

Host

Rabbit

Reactivity

Human, Monkey

Immunogen

Amino acids N2093-N2537 from the human protein were used as the immunogen for the mTOR antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Limitations

This mTOR antibody is available for research use only.

Storage Conditions

Store the mTOR antibody at -20°C.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the mTOR antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human colon carcinoma with mTOR antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.