BHC80 Antibody / PHF21A

PHD finger protein 21A is a protein that in humans is encoded by the PHF21A gene. The PHF21A gene encodes BHC80, a component of a BRAF35/histone deacetylase complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) .

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q96BD5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids M1-Q97 from the human protein were used as the immunogen for the BHC80 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Limitations

This BHC80 antibody is available for research use only.

Storage Conditions

Store the BHC80 antibody at -20°C.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the BHC80 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human sarcoma with BHC80 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.