TTC39B Antibody / C9orf52

Tetratricopeptide repeat protein 39B is a protein that in humans is encoded by the TTC39B gene. TTC39B protein contains two TPR repeats (aa 39 3-426 and 626 -659) . TCC39B protein is the product of a high density lipoprotein (HDL) gene that promotes the ubiquitination and degradation of liver X receptor (LXR) . TTC39B null or deficient mice challenged with high fat/cholesterol/bile salt diet exhibit increased LX R protein and target gene expression. TTC39B deficiency is reported to stabilize LXR by reducing its polyubiquitination and proteasomal degradation. Hence it reduces the incidence of atherosclerosis and steatohepatitis. TTC39B deficiency mice display higher levels of HDL and Apoplipoprotein A-1 and lower levels of hepatic triglyceride synthesis.However it does not affect glucose tolerance or hepatic gluconeogenesis.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q5VTQ0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids K129-D682) was used as the immunogen for the TTC39B antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This TTC39B antibody is available for research use only.

Storage Conditions

After reconstitution, the TTC39B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TTC39B antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of fixed and permeabilized human HepG2 cells with TTC39B antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= TTC39B antibody.