Leukotriene A-4 hydrolase Antibody / LTA4H

The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD) . Several transcript variants encoding different isoforms have been found for this gene.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

P09960

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids Q137-D611) was used as the immunogen for the Leukotriene A-4 hydrolase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Leukotriene A-4 hydrolase antibody is available for research use only.

Storage Conditions

After reconstitution, the Leukotriene A-4 hydrolase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Leukotriene A-4 hydrolase antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

Immunofluorescent staining of FFPE human intestinal cancer tissue with Leukotriene A-4 hydrolase antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.