PAICS Antibody / AIRC / ADE2

This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

P22234

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (amino acids Q69-L425) was used as the immunogen for the PAICS antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This PAICS antibody is available for research use only.

Storage Conditions

After reconstitution, the PAICS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PAICS antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of fixed and permeabilized human PC-3 cells with PAICS antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= PAICS antibody.