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Phospho-MCM2 Antibody pS27

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. [UniProt]

Product Specifications

Specifications

Western blot: 1:500-1:2000

UniProt

P49736

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide from the sequence surrounding phosphorylated serine 27 of human MCM2 protein was used as the immunogen for the phospho-MCM2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

28M65

Applications

WB

Purity

Affinity chromatography

Format

Purified

Limitations

This phospho-MCM2 antibody is available for research use only.

Storage Conditions

Store the phospho-MCM2 antibody at -20°C.

Product Datasheet

https://www.nsjbio.com/tds-pdf/phospho-mcm2-antibody-ps27-28m65-rq8511

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the phospho-MCM2 antibody should be determined by the researcher.

Image Legend

Western blot testing of lysate from 1) human HeLa cells and 2) human HeLa cells treated with alkaline phosphatase, using phospho-MCM2 antibody.

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