NUMB Antibody

Protein numb homolog is a protein that in humans is encoded by the NUMB gene. It is mapped to 14q24.2 to q24.3. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The primary function of Numb in cell differentiation is as an inhibitor of Notch signaling which is essential for maintaining self-renewal potential in stem and progenitor cells. Numb also plays a crucial role in asymmetrical cell division during development, allowing for differential cell fate specification in the central and peripheral nervous systems. Whatï¿½s more, the numb gene protein product controls binary cell fate decisions in the peripheral and central nervous systems of both invertebrates and mammals during neurogenesis.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

P49757

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids E59-A567) was used as the immunogen for the NUMB antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This NUMB antibody is available for research use only.

Storage Conditions

After reconstitution, the NUMB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NUMB antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of fixed and permeabilized human RT4 cells with NUMB antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= NUMB antibody.