AF10 Antibody / MLLT10

Protein AF-10 is a protein that in humans is encoded by the MLLT10 gene. This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

P55197

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids E541-S1068) was used as the immunogen for the AF10 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This AF10 antibody is available for research use only.

Storage Conditions

After reconstitution, the AF10 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the AF10 antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human A549 cells with AF10 antibody (red) and Beta Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.