LGI4 Antibody / Leucine-rich repeat LGI family member 4

The leucine-rich, glioma inactivated gene 4 (LGI4) is a member of the LGI family. The LGI family consists of four of highly related proteins containing leucine-rich repeats (LRRs) . Despite its high homology with LGI1 and similar pattern of expression, mutations in LGI4 have not been found to be associated with autodominant lateral temporal epilepsy (ADTLE) . However, the LGI4 gene is located in a region linked to benign familial infantile convulsions and a GC-to-AT polymorphism in LGI4 was correlated with childhood absence epilepsy. Other studies showed that decreasing LGI4 expression in cultured cells inhibits myelination, indicating that LGI4 may play a role in neural development.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q8N135

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids E225-H531) was used as the immunogen for the LGI4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This LGI4 antibody is available for research use only.

Storage Conditions

After reconstitution, the LGI4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the LGI4 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human liver cancer tissue with LGI4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.