Josephin 2 Antibody / JOSD2

JOSD2 (Josephin-2) is a member of the Machado-Joseph Domain (MJD) deubiquitinating enzyme family. Other family members include: Ataxin-3, Ataxin-3L, and JOSD1. JOSD2 preferentially cleaves K63-linked ubiquitin chains, with much weaker activity toward K48-linked ubiquitin chains in vitro.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q8TAC2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids M1-D188) was used as the immunogen for the Josephin 2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Josephin 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Josephin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Josephin 2 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human thyroid papillary carcinoma tissue with Josephin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.