IMP4 Antibody / U3 small nucleolar ribonucleoprotein protein IMP4

U3 small nucleolar ribonucleoprotein protein IMP4 is a protein that in humans is encoded by the IMP4 gene. The protein encoded by this gene, along with IMP3 and MPP10, is part of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP) complex. This complex is necessary for the early cleavage steps of pre-18S ribosomal RNA processing. Several transcript variants encoding different isoforms have been found for this gene.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q96G21

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids L2-K236) was used as the immunogen for the IMP4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This IMP4 antibody is available for research use only.

Storage Conditions

After reconstitution, the IMP4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the IMP4 antibody should be determined by the researcher.

Location

Nucleolus

Image Legend

Immunofluorescent staining of FFPE human HeLa cells with IMP4 antibody (green), Beta Tubulin mAb (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.