RAB3IP Antibody / Rab3A-interacting protein

RAB3A-interacting protein is a protein that in humans is encoded by the RAB3IP gene. RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport, but not for exocytosis of discoidal/fusiform-shaped vesicles.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q96QF0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids F25-R426) was used as the immunogen for the RAB3IP antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This RAB3IP antibody is available for research use only.

Storage Conditions

After reconstitution, the RAB3IP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the RAB3IP antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human liver cancer tissue with RAB3IP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.