LHPP Antibody / Phospholysine phosphohistidine inorganic pyrophosphate phosphatase

LHPP, also known as HDHD2B, is a 270 amino acid protein that exists as a homodimer and is a member of the HAD-like hydrolase superfamily. Expressed in liver, kidney and moderately in brain, LHPP is encoded by a gene located on 10, which houses over 1,200 genes and comprises nearly 4.5 % of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman?s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q9H008

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids K6-K270) was used as the immunogen for the LHPP antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This LHPP antibody is available for research use only.

Storage Conditions

After reconstitution, the LHPP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the LHPP antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

Immunofluorescent staining of FFPE human U-2 OS cells with LHPP antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.