PAIP1 Antibody / Polyadenylate-binding protein-interacting protein 1

Polyadenylate-binding protein-interacting protein 1 is a protein that in humans is encoded by the PAIP1 gene. The protein encoded by this gene interacts with poly (A) -binding protein and with the cap-binding complex eIF4A. It is involved in translational initiation and protein biosynthesis. Overexpression of this gene in COS7 cells stimulates translation. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q9H074

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (amino acids R92-Q420) was used as the immunogen for the PAIP1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This PAIP1 antibody is available for research use only.

Storage Conditions

After reconstitution, the PAIP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PAIP1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunofluorescent staining of FFPE human HeLa cells with PAIP1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.