PARP3 Antibody / ARTD3

Poly [ADP-ribose] polymerase 3 is an enzyme that in humans is encoded by the PARP3 gene. The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly (ADP-ribosyl) transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

Q9Y6F1

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

An E.coli-derived human recombinant protein (amino acids Q162-Q498) was used as the immunogen for the PARP3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This PARP3 antibody is available for research use only.

Storage Conditions

After reconstitution, the PARP3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PARP3 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human HeLa cells with PARP3 antibody (green) and Beta Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.