EIF3B Antibody / Eukaryotic translation initiation factor 3 subunit B
EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B), also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al. (1997) identified a cDNA encoding a human Prt1 homolog. Asano et al. (1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of 2 proteins, p116 (PRT1) and an unrelated 110-kD protein. Chaudhuri et al. (1997) isolated cDNAs encoding PRT1, which they called p110 based on the size of the corresponding component of rabbit eIF3.
Product Specifications
Specifications
UniProt
P55884
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An E.coli-derived human recombinant protein (amino acids Q178-K595) was used as the immunogen for the EIF3B antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, ELISA
Purity
Antigen affinity chromatography
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Limitations
This EIF3B antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the EIF3B antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
IHC staining of FFPE human colon tissue with EIF3B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Frequently Asked Questions
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