HENMT1 Antibody / HEN1 methyltransferase homolog 1

Small RNA 2'-O-methyltransferase/HENMT1 is a methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q5T8I9

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

An E.coli-derived human recombinant protein (amino acids Q45-D388) was used as the immunogen for the HENMT1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This HENMT1 antibody is available for research use only.

Storage Conditions

After reconstitution, the HENMT1 Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HENMT1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunofluorescent staining of FFPE human HeLa cells with HENMT1 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.