ARID domain-containing protein 1A Antibody / ARID1A

ARID domain-containing protein 1A is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology) . Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. [UniProt]

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

O14497

Host

Rabbit

Reactivity

Human, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids R857-Q1552) was used as the immunogen for the ARID domain-containing protein 1A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This ARID domain-containing protein 1A antibody is available for research use only.

Storage Conditions

After reconstitution, the ARID domain-containing protein 1A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ARID domain-containing protein 1A antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human lung cancer tissue with ARID domain-containing protein 1A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.