HELLS Antibody / PASG / Lymphoid-specific helicase

Lymphoid-specific helicase (Lsh), also called PASG and SMARCA6, is a member of the SNF2 helicase family of chromatin remodeling proteins that in humans is encoded by the HELLS gene. This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q9NRZ9

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

An E.coli-derived human recombinant protein (amino acids N167-F838) was used as the immunogen for the HELLS antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This HELLS antibody is available for research use only.

Storage Conditions

After reconstitution, the HELLS Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HELLS antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human HeLa cells with HELLS antibody (red) and Beta Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.