Phospho-Nucleolin Antibody (pT84)

Nucleolin is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles.

Product Specifications

Specifications

Western blot: 1:500-1:2000

UniProt

P19338

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to the region of human Nucleolin protein surrounding phosphorylated threonine 84 was used as the immunogen for the Phospho-Nucleolin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

29N17

Applications

Purity

Affinity chromatography

Format

Purified

Limitations

This Phospho-Nucleolin antibody is available for research use only.

Storage Conditions

Store the Phospho-Nucleolin antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Phospho-Nucleolin antibody should be determined by the researcher.

Image Legend

Western blot testing of lysate from human K562 cells, untreated (1) and Calyculin A treated (2), with Phospho-Nucleolin antibody.

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