ALOX12 Antibody / 12S-lipoxygenase

ALOX12, Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in situ hybridization, the ALOX12 gene is located in band 17p13.1. The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNA Arachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12 (S) -hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

P18054

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids A186-R628) was used as the immunogen for the ALOX12 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This ALOX12 antibody is available for research use only.

Storage Conditions

After reconstitution, the ALOX12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ALOX12 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human A549, 2) human MCF7, 3) human Jurkat, 4) rat RH35 and 5) mouse RAW264.7 cell lysate with ALOX12 antibody. Predicted molecular weight ~76 kDa.