Interferon regulatory factor 4 Antibody / IRF4 / MUM1

Interferon regulatory factor 4 (IRF4), also known as Multiple myeloma oncogene 1 (MUM1), is a protein that in humans is encoded by the IRF4 gene. It is located on 6p25.3. IRF4 is a transcription factor, and it is essential for the development of T helper-2 (Th2) cells, IL17 -producing Th17 cells, and IL9 -producing Th9 cells. In melanocytic cells, the IRF4 gene may be regulated by MITF. IRF4 is a transcription factor that has been implicated in acute leukemia. This gene is strongly associated with pigmentation, sensitivity of skin to sun exposure, freckles, blue eyes, and brown hair color. What's more, IRF4 inhibition is toxic to myeloma cell lines, regardless of transforming oncogenic mechanism.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

Q15306

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (amino acids A163-Q419) was used as the immunogen for the Interferon regulatory factor 4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Interferon regulatory factor 4 antibody is available for research use only.

Storage Conditions

After reconstitution, the Interferon regulatory factor 4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Interferon regulatory factor 4 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunofluorescent staining of FFPE human A549 cells with Interferon regulatory factor 4 antibody (red) and Phalloidin-iFluor 488 (green) . HIER: steam section in pH6 citrate buffer for 20 min.