SIM2 Antibody / Single-minded homolog 2 / BHLHE15

Single-minded homolog 2 is a protein that in humans is encoded by the SIM2 gene. This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q14190

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids Y337-R609) was used as the immunogen for the SIM2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This SIM2 antibody is available for research use only.

Storage Conditions

After reconstitution, the SIM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SIM2 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of fixed and permeabilized human PC-3 cells with SIM2 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= SIM2 antibody.