Phospho-MDM2 Antibody (pS166)

Cellular stress signals such as DNA damage or growth factor stimulation can trigger phosphorylation of MDM2, altering its ability to degrade p53 and impacting cell cycle progression, DNA repair, and apoptosis. Employing a Phospho-MDM2 antibody enables detection of these phosphorylation-dependent changes and provides insights into signaling cascades that control cell fate. NSJ Bioreagents offers a high-quality Phospho-MDM2 antibody for applications including western blot, immunoprecipitation, and immunofluorescence. Selecting the right Phospho-MDM2 antibody ensures reliable detection of this critical regulatory modification in cancer biology, cell cycle control, and stress response studies.

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunofluorescence: 1:50, Immunohistochemistry (FFPE) : 1:50

UniProt

Q00987

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide specific to the region of human MDM2 protein surrounding phosphorylated serine 166 was used as the immunogen for the Phospho-MDM2 antibody (pS166) .

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

29M35

Applications

WB, IF, IHC-P

Purity

Affinity chromatography

Format

Purified

Limitations

This Phospho-MDM2 antibody is available for research use only.

Storage Conditions

Store the Phospho-MDM2 antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Phospho-MDM2 antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human HeLa cells with Phospho-MDM2 antibody (green), phalloidin (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.