Cytokeratin 4 Antibody / KRT4

Cytokeratin 4 (KRT4) is an intermediate filament protein that is expressed in suprabasal cells of non-keratinized stratified squamous epithelium of esophagus, cornea, anus, larynx, pharynx and tongue.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

P19013

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (amino acids N170-S427) was used as the immunogen for the Cytokeratin 4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Cytokeratin 4 antibody is available for research use only.

Storage Conditions

After reconstitution, the Cytokeratin 4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Cytokeratin 4 antibody should be determined by the researcher.

Location

Cell membrane, cytoplasm, nucleus

Image Legend

IHC staining of FFPE human lung adenocarcinoma tissue with Cytokeratin 4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.