NUDT15 Antibody / Nudix hydrolase 15

Nudix hydrolase 15 is a protein that in humans is encoded by the NUDT15 gene. This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

Q9NV35

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (amino acids C23-L164) was used as the immunogen for the NUDT15 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This NUDT15 antibody is available for research use only.

Storage Conditions

After reconstitution, the NUDT15 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NUDT15 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunofluorescent staining of FFPE human A549 cells with NUDT15 antibody (red) and Phalloidin-iFluor 488 conjugate (green) . HIER: steam section in pH6 citrate buffer for 20 min.