GABA Transporter 1 Antibody / GAT-1 / SLC6A1

GABA transporter 1 (GAT1), also known as sodium- and chloride-dependent GABA transporter 1, is a protein that in humans is encoded by the SLC6A1 gene. GABA Transporter 1 uses Na+ and Cl- to create a gradient, which removes or adds GABA to extracellular spaces in the cerebrum and cerebellum. The stoichiometry for GABA Transporter 1 is 2 Na+: 1 Cl-: 1 GABA. The activity of GAT1 is largely dependent on the presence of Na+, while Cl- assists by increasing the ability for GAT-1 to uptake GABA.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

P30531

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids A23-A215) was used as the immunogen for the GABA Transporter 1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This GABA Transporter 1 antibody is available for research use only.

Storage Conditions

After reconstitution, the GABA Transporter 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the GABA Transporter 1 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human HeLa, 2) rat C6, and 3) mouse Neuro 2a cell lysate with SLC6A1 antibody. Predicted molecular weight is approximately 67 kDa, but SLC6A1 routinely migrates at approximately 50 to 55 kDa on SDS PAGE due to its multi pass membrane structure and glycosylation dependent mobility.