HCRT Antibody / Hypocretin neuropeptide precursor

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

O43612

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide specific to human HCRT protein was used as the immunogen for the HCRT antibody. The sequence is 100% homolgous in mouse and rat.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, IF

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This HCRT antibody is available for research use only.

Storage Conditions

After reconstitution, the HCRT Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HCRT antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE rat brain tissue with HCRT antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.