PDE12 Antibody / Mitochondrial deadenylase / 2',5'-phosphodiesterase 12

PDE12 is an enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. It also cleaves 3',5'-phosphodiester bond of oligoadenylates and plays a role as a negative regulator of the The 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs) . Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q6L8Q7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids E36-K607) was used as the immunogen for the PDE12 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This PDE12 antibody is available for research use only.

Storage Conditions

After reconstitution, the PDE12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PDE12 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) rat brain, 2) rat thymus, 3) rat PC-12, 4) mouse brain, 5) mouse thymus and 6) mouse NIH 3T3 cell with PDE12 antibody. Predicted molecular weight ~67 kDa.