PCCA Antibody / Propionyl-CoA carboxylase alpha
The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Specifications
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
P05165
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An E.coli-derived human recombinant protein (amino acids E403-E728) was used as the immunogen for the PCCA antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, ELISA
Purity
Antigen affinity chromatography
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Limitations
This PCCA antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PCCA antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
IHC staining of FFPE human liver cancer tissue with PCCA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Frequently Asked Questions
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