PCCA Antibody / Propionyl-CoA carboxylase alpha

The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

P05165

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids E403-E728) was used as the immunogen for the PCCA antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This PCCA antibody is available for research use only.

Storage Conditions

After reconstitution, the PCCA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PCCA antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human liver cancer tissue with PCCA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.