Cyclooxygenase 2 Antibody / COX-2 / PTGS2

Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z) -eicosatetraenoate, AA, C20:4 (n-6) ), with a particular role in the inflammatory response. [UniProt]

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

P35354

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

An E.coli-derived human recombinant protein (amino acids A18-L604) was used as the immunogen for the Cyclooxygenase 2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.05% sodium azide

Limitations

This Cyclooxygenase 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Cyclooxygenase 2 Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Cyclooxygenase 2 antibody should be determined by the researcher.

Location

Cytoplasm, Cell surface

Image Legend

IHC staining of FFPE human lung cancer tissue with Cyclooxygenase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.