RMI2 Antibody / BLAP18 / RecQ-mediated genome instability protein 2

RMI2 is a component of the BLM (RECQL3) complex, which plays a role in homologous recombination-dependent DNA repair and is essential for genome stability. This gene is mapped to 16p13.13. RMI1 and RMI2 were present in approximately stoichiometric amounts with other BLM complex components, including topoisomerase-3-alpha (TOP3A), RPA (see RPA1), and BLAP250. RMI2 also associated with RMI1 and TOP3A in a second complex. RMI1 and RMI2 interacted directly, and both were essential for stability of the BLM complex. Depletion of either RMI1 or RMI2 depleted the other protein by 80 to 90%. Chicken DT40 cells depleted of Rmi2 displayed elevated sister chromatid exchange, but other functions of the BLM complex appeared intact. Mutation analysis revealed that interaction between human RMI2 and BLM was essential for suppression of sister chromatid exchange.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

Q96E14

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids L23-P147) was used as the immunogen for the RMI2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This RMI2 antibody is available for research use only.

Storage Conditions

After reconstitution, the RMI2 Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the RMI2 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human 293T, 2) human MCF7, 3) human HT-1080, 4) human HeLa, 5) rat thymus and 6) mouse thymus tissue lysate with RMI2 antibody. Expected molecular weight ~18 kDa.