MACROH2A1 Antibody / Core histone macro-H2A.1 / H2AFY

Core histone macro-H2A.1 is a protein that in humans is encoded by the MACROH2A1 gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

O75367

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids A180-K301) was used as the immunogen for the MACROH2A1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This MACROH2A1 antibody is available for research use only.

Storage Conditions

After reconstitution, the MACROH2A1 Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MACROH2A1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human lung adenocarcinoma tissue with MACROH2A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.