Phospho-mTOR Antibody (pS2448)

Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins and functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2) . [UniProt]

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200

UniProt

P42345

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to the region of human mTOR protein surrounding phosphorylated serine 2448 was used as the immunogen for the Phospho-mTOR antibody (pS2448) .

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

IFF-13

Applications

WB, IHC-P

Limitations

This Phospho-mTOR antibody is available for research use only.

Storage Conditions

Store the Phospho-mTOR antibody at -20°C.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Phospho-mTOR antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human breast cancer tissue with Phospho-mTOR antibody antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.