HAAO Antibody / 3-hydroxyanthranilate 3, 4-dioxygenase / 3HAO

3-hydroxyanthranilate 3,4-dioxygenase (EC 1.13.11.6) is an enzyme encoded by the HAAO gene. 3-Hydroxyanthranilate 3,4-dioxygenase is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

P46952

Host

Rabbit

Reactivity

Human, Rat

Immunogen

An E.coli-derived human recombinant protein (amino acids E52-G286) was used as the immunogen for the HAAO antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This HAAO antibody is available for research use only.

Storage Conditions

After reconstitution, the HAAO Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HAAO antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunofluorescent staining of FFPE human Caco-2 cells with HAAO antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.