Puratrophin 1 Antibody / PLEKHG4

Puratrophin 1 is a protein that in humans is encoded by the PLEKHG4 gene. The protein encoded by this gene can function as a guanine nucleotide exchange factor (GEF) and may play a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Polymorphisms in the region of this gene have been found to be associated with spinocerebellar ataxia in some study populations. Alternative splicing results in multiple transcript variants.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q58EX7

Host

Rabbit

Reactivity

Human

Immunogen

An E.coli-derived human recombinant protein (amino acids R22-E1140) was used as the immunogen for the Puratrophin 1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, ELISA

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Puratrophin 1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Puratrophin 1 Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Puratrophin 1 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with Puratrophin 1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= Puratrophin 1 antibody.