ARHGEF12 Antibody / LARG

ARHGEF12 is widely expressed in multiple tissue types and has been implicated in various physiological and pathological conditions, such as embryonic development, wound healing, and cancer progression. Alternative splicing of the ARHGEF12 gene gives rise to several isoforms that may vary in function and cellular localization, making precise detection and characterization essential in research. A high-quality ARHGEF12 antibody is a critical tool for investigating the diverse roles of this protein in cellular signaling and disease. The ARHGEF12 antibody can be used in western blotting to evaluate protein levels, in immunofluorescence to assess subcellular localization, and in immunohistochemistry for tissue-specific expression profiling. NSJ Bioreagents offers an extensively validated ARHGEF12 antibody designed to provide specificity and reproducibility across multiple experimental platforms.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3 µg/mL, Direct ELISA: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate

UniProt

Q9NZN5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids 10-1426) was used as the immunogen for the ARHGEF12 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P, IF, IP, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This ARHGEF12 antibody is available for research use only.

Storage Conditions

After reconstitution, the ARHGEF12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ARHGEF12 antibody should be determined by the researcher.

Location

Cytoplasm, cell membrane

Image Legend

IHC staining of FFPE human liver cancer tissue with ARHGEF12 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.