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AqCbl reductase Antibody / Aquacobalamin reductase / MTRR

Dysregulation of AqCbl reductase activity can disrupt cobalamin homeostasis and is associated with metabolic disorders, including methylmalonic acidemia and homocystinuria. Given its pivotal role in cobalamin processing, AqCbl reductase has emerged as a critical marker in studies of vitamin B12 metabolism and related diseases. Researchers have also explored its function in oxidative stress responses and mitochondrial health. The AqCbl reductase antibody enables specific detection of this enzyme in biological samples, supporting studies of cobalamin-dependent metabolic pathways. It is widely used in western blot, immunohistochemistry, and ELISA applications to investigate tissue-specific expression patterns and disease-related changes. With proven performance and high specificity, the AqCbl reductase antibody is an indispensable tool for scientists exploring vitamin B12 metabolism. Incorporating the AqCbl reductase antibody into your research workflow provides valuable insights into the regulation and function of this essential metabolic enzyme.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3 µg/mL, Direct ELISA: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate

UniProt

Q9UBK8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids L7-S698) was used as the immunogen for the AqCbl reductase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This AqCbl reductase antibody is available for research use only.

Storage Conditions

After reconstitution, the AqCbl reductase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the AqCbl reductase antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunofluorescent staining of FFPE human stomach tissue with AqCbl reductase antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.
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