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ME1 Antibody / Malic enzyme 1

ME1 is expressed in various tissues, with particularly high levels in lipogenic tissues such as liver, adipose tissue, and lactating mammary gland. Its activity is regulated by nutritional and hormonal signals, including carbohydrate intake and insulin, making it a key player in metabolic adaptation. Due to its involvement in energy metabolism and biosynthetic processes, ME1 has been widely studied in the contexts of metabolic regulation and biochemical pathway integration. The ME1 antibody is a valuable reagent for detecting ME1 in research applications such as western blot, immunohistochemistry, and immunofluorescence. Researchers use the ME1 antibody from NSJ Bioreagents to assess tissue-specific expression, study its role in lipid metabolism, and investigate its contribution to NADPH production. With strong specificity and reproducible performance, the ME1 antibody supports a wide range of studies in cellular metabolism, enzymology, and biochemical research. ME1 antibody

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P48163

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids M1-Q572) was used as the immunogen for the ME1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This ME1 antibody is available for research use only.

Storage Conditions

After reconstitution, the ME1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ME1 antibody should be determined by the researcher.

Location

Cytoplasm, nucleus, cell membrane

Image Legend

Immunofluorescent staining of FFPE human gastric cancer tissue with ME1 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.

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