GAR1 Antibody / H/ACA ribonucleoprotein complex subunit 1 / NOLA1

GAR1 interacts with other snoRNP core proteins, including DKC1, NOP10, and NHP2, to ensure stability and catalytic activity of the H/ACA complex. Beyond its role in rRNA processing, GAR1 has been implicated in telomerase function and the regulation of RNA stability. Disruption of GAR1 activity has been associated with ribosomopathies and defects in cellular proliferation. Using a high-quality GAR1 antibody enables accurate detection in applications such as western blot, immunohistochemistry, and immunofluorescence. A GAR1 antibody from NSJ Bioreagents provides sensitivity and reproducibility for research in RNA modification, ribosome assembly, and nucleolar biology. Selecting the right GAR1 antibody is essential for generating reliable and consistent results.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2ug per 500ug of lysate, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q9NY12

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids F58-K165) was used as the immunogen for the GAR1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, IP, IHC-P

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This GAR1 antibody is available for research use only.

Storage Conditions

After reconstitution, the GAR1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the GAR1 antibody should be determined by the researcher.

Location

Nucleolus

Image Legend

Immunofluorescent staining of FFPE human MCF7 cells with GAR1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.