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MED7 Antibody / Mediator of RNA polymerase II transcription subunit 7

Selected Variant

RQ8912

Size: 100 µg

MED7 is ubiquitously expressed and contributes to the regulation of genes involved in development, cell cycle progression, and differentiation. Dysregulation of Mediator subunits, including MED7, has been linked to transcriptional misregulation in cancer and developmental disorders, making it an important target in transcriptional biology research. Using a high-quality MED7 antibody allows sensitive detection in applications such as western blot, immunohistochemistry, and chromatin immunoprecipitation. A MED7 antibody from NSJ Bioreagents ensures specificity and reproducibility for studies focused on transcriptional control, Mediator complex biology, and disease mechanisms. Selecting the right MED7 antibody is essential for generating consistent and meaningful data.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate

UniProt

O43513

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids M1-P233) was used as the immunogen for the MED7 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, IP

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This MED7 antibody is available for research use only.

Storage Conditions

After reconstitution, the MED7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MED7 antibody should be determined by the researcher.

Location

Nucleolus

Image Legend

Immunofluorescent staining of FFPE human A549 cells with MED7 antibody (red) and Beta Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.

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