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NES Antibody / Nestin

In addition to the nervous system, NES is expressed in other progenitor cell types and is re-expressed in certain pathological conditions, including cancer and tissue injury. Its dynamic regulation reflects cellular plasticity and proliferation. Employing a NES antibody enables the detection of stem cell populations and the study of disease-associated re-expression. NSJ Bioreagents provides a high-quality NES antibody validated for immunohistochemistry, immunofluorescence, and western blot. Using a NES antibody ensures sensitive and reproducible detection in research focused on stem cells, neurobiology, and pathology.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1:50, Western blot: 1:500, Immunofluorescence: 1:50

UniProt

P48681

Host

Rabbit

Reactivity

Human, Mouse, Rat, Pig

Immunogen

A peptide sequence specific to Nestin protein was used as the immunogen for the NES antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

FBH-14

Applications

IHC-P, WB, IF

Purity

Affinity chromatography

Format

Purified

Limitations

This NES antibody is available for research use only.

Storage Conditions

After reconstitution, the NES antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the NES antibody should be determined by the researcher.

Location

Cytoplasm, membrane

Image Legend

Immunofluorescent staining of FFPE rat brain tissue with NES antibody. HIER: steam section in pH8 EDTA buffer for 20 min.

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