Hephaestin Antibody / HEPH

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q9BQS7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant partial protein (amino acids E348-Q1157) was used as the immunogen for the Hephaestin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Hephaestin antibody is available for research use only.

Storage Conditions

After reconstitution, the Hephaestin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Hephaestin antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human small intestine tissue with Hephaestin antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.