Zebrafish Nedd8 Antibody / Nedd8L

NEDD8 is a protein that in humans is encoded by the NEDD8 gene. Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The most substrates of NEDD8 modification are the Cullin subunits of Cullin-based E3 ubiquitin ligases, which are active only when neddylated. Their NEDDylation is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

F1QMF9, Q6DGU4

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Nedd8/8L recombinant protein (amino acids M1-G76) was used as the immunogen for the Zebrafish Nedd8 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Nedd8L antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Nedd8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Location

Cytoplasm, Nucleus

Image Legend

Immunohistochemical analysis of Nedd8/8L protein using Nedd8 antibody and paraffin-embedded zebrafish spinal cord tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.